Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Cytotoxicity of berberine and d-limonene, alone and in combination, on the growth of MGC803 cells. MGC803 cells were treated with berberine (C, D) and d-limonene (E, F), alone and in combination (G, H), at 1: 4, for 24 (C, E, G) and 48 h (D, F, H), with cisplatin (A for 24 h, B for 48 h) as a positive control. The viability of cells was qualified by MTT assay, reported as fraction affected (fraction of cells affected by treatment), and data represent the mean±SD from three replicate wells. P<.01, compared with the control group.

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Positive Control, MTT Assay, Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Synergistic anticancer effect of the combination of berberine and d-limonene in MGC803 cells. The combination index (CI) (A, B) and dose-reduction index (DRI) (C, D) of the combination of berberine and d-limonene were calculated as described. The data points below CI values of 1, denoted by horizontal dotted lines in (A) and (B), are indicative of synergistic effects.

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques:

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Cell-Cycle Distribution Induced by Berberine and d -Limonene Alone and in Combination in MGC803 Cells

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Induction apoptosis of berberine and d-limonene, alone and in combination, in MGC803 cells. Morphological changes were shown by acridine orange after drug treatment for 48 h: (A) control, (B) 20 μM cisplatin, (C) 20 μM berberine, (D) 80 μM d-limonene, and (E) 20 μM berberine+80 μM d-limonene. The apoptosis induced by berberine and d-limonene at 24, 36, and 48 h was determined by flow cytometry (F). Data are mean±SD values. P<.05, asignificantly different compared with the control group; bsignificantly different compared with berberine group; csignificantly different compared with d-limonene group. The bar is 25 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Control, Flow Cytometry

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Induction of intracellular reactive oxygen species (ROS) generation in MGC803 cells after treatment with berberine and d-limonene, alone and in combination, for 24 and 48 h. Monitoring of the ROS production with DCF (A) in MGC803 cells; fluorescence intensity (FI) was measured by laser scanning confocal microscopy. Data presented as mean±SD values of three independent experiments (B). P<.05, asignificantly different compared with the control group; bsignificantly different compared with the single drug treatments. The bar is 15 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Fluorescence, Confocal Microscopy, Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: Loss of mitochondrial membrane potential (ΔΨm) in MGC803 cells after treatment with berberine and d-limonene, alone and in combination, for 24 and 48 h. Detection of ΔΨm changes with Rhodamine 123 (A) in MGC803 cells; FI was measured by laser scanning confocal microscopy. Data presented as mean±SD values of three independent experiments (B). P<.05, asignificantly different compared with the control group; bsignificantly different compared with the single drug treatments. The bar is 15 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Membrane, Confocal Microscopy, Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: The changes of Bcl-2 protein expression in MGC803 cells after treatment with berberine and d-limonene, alone and in combination, for 24 and 48 h. The expression of Bcl-2 in MGC803 cells was detected by the FITC-conjugated secondary antibody (A), and FI was measured by laser scanning confocal microscopy. Data presented as mean±SD values of three independent experiments (B). P<.05, asignificantly different compared with the control group; bsignificantly different compared with the single drug treatments. The bar is 15 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Expressing, Confocal Microscopy, Control

Journal: Journal of Medicinal Food

Article Title: Synergistic Inhibitory Effect of Berberine and d -Limonene on Human Gastric Carcinoma Cell Line MGC803

doi: 10.1089/jmf.2013.2967

Figure Lengend Snippet: The changes in caspase-3 protein expression in MGC803 cells after treatment with berberine and d-limonene, alone and in combination, for 24 and 48 h. The expression of caspase-3 (A) in MGC803 cells was detected by the FITC-conjugated secondary antibody, and FI was measured by laser scanning confocal microscopy. Data presented as mean±SD values of three independent experiments (B). P<.05, asignificantly different compared with the control group; bsignificantly different compared with the single drug treatments. The bar is 15 μm. Color images available online at www.liebertpub.com/jmf

Article Snippet: The human gastric carcinoma cell line MGC803 was purchased from China Center for Type Culture Collection.

Techniques: Expressing, Confocal Microscopy, Control

Journal: Oncology reports

Article Title: HGF derived from cancer‑associated fibroblasts promotes vascularization in gastric cancer via PI3K/AKT and ERK1/2 signaling.

doi: 10.3892/or.2018.6500

Figure Lengend Snippet: Figure 2. HGF promotes tube angiogenesis, VM formation and mosaic vessel formation. (A) Representative images of angiogenesis and VM with different treatments as indicated. Scale bars, 1,000 µm. (B) The proliferation assay of HUVECs and MGC803 cells was performed and assessed by CCK‑8 assay. (C) An in vitro Transwell co‑culture system. (D) Migration of HUVECs and MGC803 cells were performed and measured by Transwell assay. Scale bars, 200 µm. (E) HUVECs labeled with DiO (green) and MGC803 cells labeled with Dil (red) were subjected to mosaic vessel assays. Scale bars, 1,000 µm. HGF, 50 ng/ml; HGF neutralizing antibody, 300 ng/ml. **P<0.01, ***P<0.001.

Article Snippet: In brief, HUVECs and MGC803 cells were lysed using the RIPA buffer (Thermo Fisher Scientific, Inc.) after stimulation with recombinant human HGF (50 ng/ml; cat. no. ab105061; Abcam) for 1 h. In groups of inhibition, HUVECs and MGC803 cells were pretreated with LY294002 (50 μM; cat. no. 9901) and U0126 (20 μM; cat. no. 9903; both from Cell Signaling Technology, Inc., Danvers, MA, USA) for 6 h before they were co-cultured with primary CAFs for 36 h. Human HGF antibody (300 ng/ml; cat. no. 24612; R&D Systems) for neutralization and LY294002 (50 μM) U0126 (20 μM) for inhibition were added into the co-culture system.

Techniques: Proliferation Assay, CCK-8 Assay, In Vitro, Migration, Transwell Assay, Labeling

Journal: Oncology reports

Article Title: HGF derived from cancer‑associated fibroblasts promotes vascularization in gastric cancer via PI3K/AKT and ERK1/2 signaling.

doi: 10.3892/or.2018.6500

Figure Lengend Snippet: Figure 3. PI3K/AKT and ERK1/2 participate in angiogenesis and VM formation induced by HGF. (A) Representative images of co‑localization of α‑SMA and CD31 in a frozen section of a GC sample. Scale bars, 400 µm. (B) Downstream oncogenic signals were detected by western blot analysis in HUVECs and MGC803 cells with different treatments as indicated. (C) PI3K/AKT inhibitor LY294002 (50 µM) and ERK1/2 inhibitor U0126 (20 µM) decreased angiogenesis and VM formation. Scale bars, 1,000 µm. Bar charts revealed the number of tubes and intersections of (D) angiogenesis and (E) VM structures. HGF, 50 ng/ml; HGF neutralizing antibody, 300 ng/ml. **P<0.01, ***P<0.001.

Article Snippet: In brief, HUVECs and MGC803 cells were lysed using the RIPA buffer (Thermo Fisher Scientific, Inc.) after stimulation with recombinant human HGF (50 ng/ml; cat. no. ab105061; Abcam) for 1 h. In groups of inhibition, HUVECs and MGC803 cells were pretreated with LY294002 (50 μM; cat. no. 9901) and U0126 (20 μM; cat. no. 9903; both from Cell Signaling Technology, Inc., Danvers, MA, USA) for 6 h before they were co-cultured with primary CAFs for 36 h. Human HGF antibody (300 ng/ml; cat. no. 24612; R&D Systems) for neutralization and LY294002 (50 μM) U0126 (20 μM) for inhibition were added into the co-culture system.

Techniques: Western Blot

Journal: Oncology reports

Article Title: HGF derived from cancer‑associated fibroblasts promotes vascularization in gastric cancer via PI3K/AKT and ERK1/2 signaling.

doi: 10.3892/or.2018.6500

Figure Lengend Snippet: Figure 5. PI3K/AKT and ERK1/2 participate in mosaic vessel formation induced by HGF. (A) Representative images of mosaic vessels with different treat- ments as indicated. HUVECs labeled with DiO (green) and MGC803 cells labeled with Dil (red). HGF, 50 ng/ml; LY294002, 50 µM; U0126, 20 µM. Scale bars, 1,000 µm. (B) Bar charts displaying the number of tubules, the number of intersections and the number of MGC803 cells in mosaic vessels between different groups. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: In brief, HUVECs and MGC803 cells were lysed using the RIPA buffer (Thermo Fisher Scientific, Inc.) after stimulation with recombinant human HGF (50 ng/ml; cat. no. ab105061; Abcam) for 1 h. In groups of inhibition, HUVECs and MGC803 cells were pretreated with LY294002 (50 μM; cat. no. 9901) and U0126 (20 μM; cat. no. 9903; both from Cell Signaling Technology, Inc., Danvers, MA, USA) for 6 h before they were co-cultured with primary CAFs for 36 h. Human HGF antibody (300 ng/ml; cat. no. 24612; R&D Systems) for neutralization and LY294002 (50 μM) U0126 (20 μM) for inhibition were added into the co-culture system.

Techniques: Labeling

Journal: BMC Gastroenterology

Article Title: Bioinformatics analysis and in vitro studies identify COX7C as a prognostic predictor in gastric adenocarcinoma

doi: 10.1186/s12876-025-04253-8

Figure Lengend Snippet: COX7C is downregulated in STAD cell lines and is effectively regulated in MGC803 cells. A - C COX7C expression in normal and STAD cell lines (D-F) COX7C mRNA and protein levels in MGC803 cells in the COX7C overexpression group G - I COX7C mRNA and protein levels in MGC803 cells in the COX7C knocked-dwon group *: P < 0.05; **: P < 0.01; ***: P < 0.001; ***: P < 0.0001

Article Snippet: The human STAD cell line MGC803, AGS, HGC-27 and human gastric epithelial cells GSE-1 (immocell, Xiamen, China) was cultured in roswell park memorial institute 1640 (RPMI-1640) medium (Proteintech, Hubei, China) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin-streptomycin (Gibco, USA).

Techniques: Expressing, Over Expression

Journal: BMC Gastroenterology

Article Title: Bioinformatics analysis and in vitro studies identify COX7C as a prognostic predictor in gastric adenocarcinoma

doi: 10.1186/s12876-025-04253-8

Figure Lengend Snippet: Effects of COX7C overexpression or knockdown on MGC803 cell viability, apoptosis, migration, and invasion. A Cell viability was measured using the CCK-8 assay at 48 h after transfection. Groups included untreated control, vector control (Control OE), COX7C overexpression (COX7C OE), non-targeting siRNA control (Control KD), and COX7C knockdown (COX7C KD) B Apoptosis was quantified using Annexin V-FITC/PI staining followed by flow cytometry. Apoptotic percentage includes early and late apoptotic cells C Wound closure rates were quantified 24 h after scratching in the groups as control, vector control (Control OE), COX7C overexpression (COX7C OE), non-targeting siRNA control (Control KD), and COX7C knockdown (COX7C KD). Migration ability was expressed as percentage of wound area closed relative to 0 h D Counts of cell invasion through Matrigel-coated Transwell chambers after 24 h E Apoptotic cell detection by flow cytometry. Right upper (late apoptosis) and right lower (early apoptosis) quadrants represent apoptotic populations F Wound healing images at 24 h post-scratch G Representative images corresponding to the Transwell invasion assay OE: overexpression; KD: knockdown; SD: standard deviation; FITC: fluorescein isothiocyanate; PI: propidium iodide; CCK-8: Cell Counting Kit-8. ** P < 0.01

Article Snippet: The human STAD cell line MGC803, AGS, HGC-27 and human gastric epithelial cells GSE-1 (immocell, Xiamen, China) was cultured in roswell park memorial institute 1640 (RPMI-1640) medium (Proteintech, Hubei, China) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin-streptomycin (Gibco, USA).

Techniques: Over Expression, Knockdown, Migration, CCK-8 Assay, Transfection, Control, Plasmid Preparation, Staining, Flow Cytometry, Transwell Invasion Assay, Standard Deviation, Cell Counting